First slide

Processes of Recombinant DNA Technology

Question

In continuous culture

Easy

A

a large biomass is produced leading to higher yields of desired protein.
B

the cells cannot be maintained in log phase for long.
C

there is continuous accumulation of undesired metabolite.
D

optimum growth conditions can not be maintained in this type of culture.

Solution

The genetically modified cells can be multiplied in a continuous culture system wherein the used medium is drained out from one side while fresh medium is added from the other to maintain the cells in their physiologically most active log/exponential phase. This type of culturing method produces a larger biomass leading to higher yields of desired protein.

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