First slide

Processes of Recombinant DNA Technology

Question

The correct order of steps in Polymerase Chain Reaction (PCR) is

Easy

A

extension, denaturation, annealing
B

annealing, extension, denaturation
C

denaturation, extension, annealing
D

denaturation, annealing, extension

Solution

Each cycle of PCR (Polymerase chain reaction) has three steps:
(i) Denaturation
(ii) Primer annealing
(iii) Extension of primers

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