First slide

The Search for Genetic Material

Question

During Messelson and Stahl experiment, heavy DNA was distinguished from normal DNA by centrifugation in

Easy

A

${SO}_{2}$
B

$^{15} {NH}_{4} Cl$
C

$^{14} {NH}_{4} Cl$
D

CsCl gradient

Solution

Meselson and Stahl subjected the DNA isolated from E.coli after certain period of incubation at the beginning in $^{15} {NH}_{4} Cl A$ and later in $^{14} {NH}_{4} Cl$ to CsCl gradient centrifugation to suspend the heavy DNA at the bottom, lighter DNA at the upper level and hybrid DNA at the median position of centrifuge

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