First slide

Tools of Recombinant DNA Technology

Question

EcoR I recognises a base sequence which has

Easy

A

3 GC pairs and 3 AT pairs
B

4 GC pairs and 2 AT pairs
C

2 GC pairs and 4 AT pairs
D

2 AT pairs and 2 GC pairs

Solution

EcoR I recognises a restriction site in DNA which has base sequence as 5^! GAATTC 3^!. Hence it has 2 GC pairs one at each end and 4 AT pairs in between these two.

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