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Q.

An E.coli cell containing hybrid DNA was placed in a medium containing heavy nitrogen (15N). Calculate the percentage of heavy, light and hybrid DNA after 2 generations in the same medium (15N).

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Detailed Solution

Initially DNA contain 15N14NThen it is placed in a medium containing 15N for 2 generation.After 1 generation:15N14Nâ 15N14N 15N15NAfter 2nd generation15N14N 15N15Nâ 15N14N 15N15N 15N15N 15N15NTherefore, we can clearly see that 75% of DNA formed is heavy and 25% of the DNA is hybrid.

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An E.coli cell containing hybrid DNA was placed in a medium containing heavy nitrogen (15N). Calculate the percentage of heavy, light and hybrid DNA after 2 generations in the same medium (15N).