Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.

Easy

A

The enzyme recognises a specific palindromic nucleotide sequence in the DNA.
B

The enzyme cuts DNA molecule at identified position within the DNA.
C

The enzyme binds DNA at specific sites and cuts only one of the two strands.
D

The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Solution

Each restriction endonuclease recognises a specific palindromic nucleotide sequences in the DNA. Each restriction endonuclease functions by ‘inspecting’ the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar -phosphate backbones.

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