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Q.

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

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a

EcoRI

b

Taq polymerase

c

polymerase III

d

ligase

answer is D.

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Detailed Solution

Foreign DNA and plasmid cut by same restriction endonuclease can be joined to form r-DNA by DNA ligase enzyme that facilate phhospho diester bond formation between them.EcoRI = is cutting enzyme not joiningTaq polymerase take part in PCR for polymerisation not for joining. Polymerase-III used by prokaryotes for polymerisation of nucleotides not for joining of DNA fragments together.
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A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using