Principles of Biotechnology
Question
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
Easy
Solution
Foreign DNA and plasmid cut by same restriction endonuclease can be joined to form r-DNA by DNA ligase enzyme that facilate phhospho diester bond formation between them.
EcoRI = is cutting enzyme not joining
Taq polymerase take part in PCR for polymerisation not for joining. Polymerase-III used by prokaryotes for polymerisation of nucleotides not for joining of DNA fragments together.
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