First slide

Tools of Recombinant DNA Technology

Question

In genetic engineering, restriction enzymes are used for

Easy

A

isolation of genomic DNA
B

isolation of gene from genomic DNA
C

incorporating rDNA into host cell
D

maintaining the heterologous host in bioreactors

Solution

Restriction endonucleases are used to cut the genomic DNA into fragments to isolate the desired gene from other genes of the genome. These are also used to open the circular vector DNA to incorporate the desired DNA fragment to form recombinant DNA or chimeric DNA.

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