First slide

The Search for Genetic Material

Question

A heavy DNA is made to replicate for two generations in ¹⁴N containing NH₄Cl. How many dsDNA molecules shall possess ¹⁵N?

Easy

A

Two
B

Four
C

Zero
D

One

Solution

Heavy DNA- N¹⁵N¹⁵
It is grown for 2 generations in ¹⁴NH₄Cl medium.
After two generation the DNA formed will have N¹⁴N¹⁴, N¹⁵N¹⁴, N¹⁴N¹⁴, N¹⁴N¹⁵.
Therefore two dsDNA possess N¹⁵ , one strand in each DNA molecule but not both the strands of DNA.

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