First slide

Processes of Recombinant DNA Technology

Question

The host bacterial cells can be made competent by treating with

Easy

A

KCl
B

NaCl
C

${CaCl}_{2}$
D

Lysozyme

Solution

Host bacterial cells can be made competent by treating the host cells with Calcium chloride, which promotes the expansion of pores of cell wall for easy entry of rDNA through cell wall. Later by thermal shock the rDNA is pulled into the cell cytoplasm through cell membrane.

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