First slide

Tools of Recombinant DNA Technology

Question

Identify the A, B, C respectively in the following diagram

Easy

A

Site of action of EcoRI, Clipping end and rDNA
B

Site action on ligase, Clipping end, rDNA
C

Site action of EcoRI, Blunt end, hybrid DNA
D

Palindromic sequence of RNA, Ligation, rDNA

Solution

A is site of action of EcoRI in between G and A of GAATTC palindromic sequence on both the strands of DNA. B is the clipping end of the fragment with overhang as AATT and C is the rDNA formed upon ligase action between two DNA fragments of different origin.

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