Identify the incorrect statement.

Moderate

A

Insertional inactivation of $β$ -galactosidase gene leads to blue colour colonies.
B

In insertional inactivation, the rDNA in inserted within the coding sequence of an enzyme β-galactosidase.
C

Selection of recombinants due to inactivation of antibiotic is a cumbersome procedure.
D

Insertional inactivation of enzyme β-galactosidase leads to colourless colonies.

Solution

Insertional inactivation is a technique that is used in recombinant DNA technology. During insertional inactivation of $β$ galactosidase gene no blue color colonies develop as beta galactosidase is not produced due to deactivation of the lacZ gene.

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