First slide

Tools of Recombinant DNA Technology

Question

Identify the restriction sites of pBR322, where if genes are inserted results in the phenomenon insertional inactivation.
I. Pvu I II. Pvu II III. Sal I IV. Pst I

Easy

A

I, II, III
B

II, III, IV
C

I, III, IV
D

I, II, IV

Solution

The restriction sites Pvu I and Pst I are present within the marker gene ${amp}^{R}$ and Sal I within another marker gene ${tet}^{R}$ . If genes are inserted at these places it results in insertional inactivation of marker genes. Since Pvu II is not present within marker genes, if a gene is inserted at this site it does not result in inactivation of expression of marker genes.

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