Q.
Identify the restriction sites of pBR322, where if genes are inserted results in the phenomenon insertional inactivation.I. Pvu I II. Pvu II III. Sal I IV. Pst I
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a
I, II, III
b
II, III, IV
c
I, III, IV
d
I, II, IV
answer is C.
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Detailed Solution
The restriction sites Pvu I and Pst I are present within the marker gene ampRand Sal I within another marker gene tetR. If genes are inserted at these places it results in insertional inactivation of marker genes. Since Pvu II is not present within marker genes, if a gene is inserted at this site it does not result in inactivation of expression of marker genes.
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