Identify the wrong statement with regard to Cloning vectors:

Easy

A

Cloning vector needs to have a large number (minimum 20) of recognition sites for the commonly used each restriction enzymes.
B

Cloning vector must have an origin of replication
C

Retroviruses are used to transfer genes into animals
D

It is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.

Solution

In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. Presence of more than one recognition site within the vector will generate several fragments, which will complicate the gene cloning.

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