First slide

Tools of Recombinant DNA Technology

Question

Identify the wrong statement with regard to Restriction Enzymes.

Moderate

A

They cut the strand of DNA at palindromic sites.
B

They are useful in genetic engineering.
C

Sticky ends can be joined by using DNA ligases.
D

Each restriction enzyme functions by inspecting the length of a DNA sequence.

Solution

For joining of sticky end as well as blunt ends DNA ligase is used while RE cut the DNA at specific locations.
Each restriction endonuclease functions by ‘inspecting’ the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar -phosphate backbones. Each restriction endonuclease recognises a specific palindromic nucleotide sequences in the DNA. Restriction endonucleases are used in genetic engineering to form ‘recombinant’ molecules of DNA, which are composed of DNA from different sources/genomes.

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