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Q.

If alien DNA is introduced using Pst I in pBR322 then the recombinant transformant can grow on :

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a

ampicillin containing medium

b

tetracyline containing medium

c

ampicillin as well as tetracyclin containing medium.

d

neither ampicillin nor tetracyclin containing medium.

answer is B.

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Detailed Solution

The Pst I restriction site is present within the ampicillin resistant gene. Therefore, if the alien DNA is inserted within the ampicillin resistant gene then it will exhibit insertional inactivation. Therefore, it will grow on a medium containing tetracyclin and fail to grow on ampicillin containing medium.

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