If E. coli having heavy DNA was allowed to grow for $$80$$ minutes on a medium containing $$14NH4Cl$$ then what would be the proportions of light and hybrid densities DNA molecule?

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Infinity Learn · Accepted Answer

$$80minutes=$$ $$80/20=$$ $$4$$ generationsInitially DNA contain $$15N15NThen$$ it is placed in a medium containing $$14N$$ for $$3$$ generation.After $$1st$$ generation:$$15N15N$$→ $$15N14N$$    $$15N14N$$ (2$$ Hybrid DNA $$molecules)After$$ $$2nd$$ $$generation15N14N$$    $$15N14N$$ → $$15N14N$$    $$14N14N$$   $$15N14N$$    $$14N14N$$ $$(2$$ light DNA and $$2$$ Hybrid DNA $$molecules)After$$ $$3rd$$ generation:$$15N14N$$    $$14N14N$$   $$15N14N$$    $$14N14N$$ → $$15N14N$$    $$14N14N$$   $$14N14N$$   $$14N14N$$ $$15N14N$$    $$14N14N$$   $$14N14N$$   $$14N14N$$ $$(6$$ light DNA and $$2$$ hybrid DNA $$molecules)After$$ $$4th$$ $$generation2(15N14N) → $$2$$ (15N14N$$    $$14N14N)6(14N14N) → $$6$$ $$(14N14N$$   $$14N14N)Total$$ no. of light $$DNA=$$ $$14Total$$ no. of Hybrid $$DNA=$$ $$2$$% of light $$DNA=$$ $$87.5$$%% of light $$DNA=$$ $$12.5$$%

If E. coli having heavy DNA was allowed to grow for 80 minutes on a medium containing 14NH4Cl then what would be the proportions of light and hybrid densities DNA molecule?

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