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If a gene is inserted in the pBR 322 plasmid by using the enzyme Bam H I, and the rDNA is incorporated into E.coli host. Identify the functions of the marker gene.

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ampRhelps in selecting the recombinants from non-recombinants and tetR helps in selecting the transformants from non-transformants.

ampR helps in selecting the transformants from non-transformants and tetR helps in selecting the recombinants from non-recombinants.

Both ampRand tetRhelp in selecting the recombinants from non-recombinants

Both ampRand tetRhelp in selecting the transformants from non-transformants

answer is B.

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Detailed Solution

If a gene is inserted in pBR322 at BamHI, site, the tetRmarker is inactivated by insertion and this one helps in identifying recombinants from non-recombinants. The other marker ampRhelps in selecting transformants from non-transformants.

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