First slide

Tools of Recombinant DNA Technology

Question

If the gene of interest is introduced in a tetracycline – resistant gene region of pBR322, then:

Easy

A

The host will become resistant for tetracycline
B

The host will start producing tetracycline
C

The host will start producing ampillicin
D

The host will loose resistance for antibiotic tetracycline

Solution

If we ligate a foreign DNA (gene of interest) at the BamH I site of tetracycline resistance gene in the vector pBR322. The recombinant plasmids will lose tetracycline resistance due to insertion of foreign DNA.

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