First slide

Tools of Recombinant DNA Technology

Question

The introduction of T-DNA into plants involves

Easy

A

exposing the plants to cold for a brief period
B

allowing the plant roots to stand in water
C

infection of the plant by Agrobacterium tumefaciens
D

altering the pH of the soil, then heat-shocking the plants

Solution

The introduction of T-DNA into plants involves infection of the plant by Agrobacterium tumefaciens
Agrobacterium tumefaciens is a causal agent of crown gall disease in several dicot plants. It is a rod shaped, gram - negative soil bacterium. The disease is caused due to the transfer of a DNA segment from the bacterium to the plant nuclear genome. The DNA segment which is transferred is called T – DNA and is part of a large Ti (tumour inducing) plasmid found in virulent strains of Agrobacterium tumefaciens. Agrobacterium infection (utilizing its plasmids as vectors) has been extensively utilized for transfer of foreign DNA into several dicotyledonous species.

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