First slide

Tissue Culture

Question

To isolate protoplast of typical plant cells, one needs to apply
a. pectinase
b. cellulase
c. chitinase

Easy

A

Only (a)
B

Only (b)
C

Both (a) and (b)
D

Both (a) and (c)

Solution

As the cell wall of plant cells consists of pectin and cellulose which can be removed by digestion with combination of enzymes pectinase and cellulase to isolate the protoplast.

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