First slide

Regulation of Gene Expression

Question

In lac operon:

Easy

A

The presence of lactose activates the repressor
B

Repressor gene is a non-constitutive gene
C

Only operator, lac z and lac y are transcribed
D

Activation of the operon cannot take place in presence glucose or galactose.

Solution

In the absence of a preferred carbon source such as glucose, if lactose is provided in the growth medium of the bacteria, the lactose is transported into the cells through the action of permease.
In the presence of an inducer, such as lactose or allolactose, the repressor is inactivated by interaction with the inducer. This allows RNA polymerase access to the promoter and transcription proceeds.
The repressor of the operon is synthesised (all-the-time – constitutively) from the i gene. The repressor protein binds to the operator region of the operon and prevents RNA polymerase from transcribing the operon.
Operator is lacks self expression. The lac i, lac z, lac y and lac a is transcribed.

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