First slide

Processes of Recombinant DNA Technology

Question

The linking of antibiotic resistance gene with the plasmid vector became possible with the enzyme.

Easy

A

DNA ligase
B

DNA polymerase
C

Restriction endonucleases
D

Polynucleotide kinase

Solution

DNA ligase is used to link antibiotic resistance gene to the vector DNA which forms phosphodiester bonds between the vector and the gene of interest at the cohesive ends.

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