First slide

Processes of Recombinant DNA Technology

Question

Lysozyme is used during the initial step of recombinant DNA technology:

Easy

A

To remove the bacterial cell wall by hydrolysis for obtaining its DNA of genome.
B

To remove RNA
C

To precipitate DNA
D

To facilitate action of restriction enzymes

Solution

In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macro-molecules. Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant tissues/fungal cells or filaments with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).

Get Instant Solutions

When in doubt download our app. Now available Google Play Store- Doubts App

Recieve an sms with download link

Doubts App

OR

SCAN ME

Doubts App

Doubts App