Lysozyme is used during the initial step of recombinant DNA technology:

see full answer

Your Exam Success, Personally Taken Care Of

1:1 expert mentors customize learning to your strength and weaknesses – so you score higher in school , IIT JEE and NEET entrance exams.

An Intiative by Sri Chaitanya

To remove the bacterial cell wall by hydrolysis for obtaining its DNA of genome.

To remove RNA

To precipitate DNA

To facilitate action of restriction enzymes

answer is A.

(Unlock A.I Detailed Solution for FREE)

Best Courses for You

JEE

NEET

Foundation JEE

Foundation NEET

CBSE

Detailed Solution

In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macro-molecules. Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant tissues/fungal cells or filaments with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).

Watch 3-min video & get full concept clarity