Match the organism with its use in biotechnology:
| Column I | Column II |
| (a) Bacillus thuringiensis | (i) Cloning vector |
| (b) Thermus aquaticus | (ii) Construction of first rDNA molecule |
| (c) Agrobacterium tumefaciens | (iii) DNA polymerase |
| (d) Salmonella typhimurium | (iv) Cry protein |
Select the correct option from the following:
Bacillus thuringiensis has a cry gene that codes for a toxic protein called Cry protein.
Thermus aquaticus is found in hot water spring. It has thermostable DNA polymerase named Taq polymerase.
Agrobacterium tumefaciens has a ti plasmid that is modified to use as cloning vector. The tumor inducing (Ti) plasmid of Agrobacterium tumifaciens has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants.
The construction of the first recombinant DNA emerged from the linking of a gene encoding antibiotic resistance with a native plasmid of Salmonella typhimurium.
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