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In order to insert alien DNA at the site of tetracycline resistance gene in pBR322 the restriction endonuclease used can be

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a

Pvu I

b

Cla I

c

Sal I

d

Pst I

answer is C.

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Detailed Solution

The tetracycline resistance gene in pBR322 contains restriction sites for BamH I and Sal I. Therefore, in order to insert alien DNA at the site of tetracycline resistance the restriction endonuclease used has to Sal I.

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In order to insert alien DNA at the site of tetracycline resistance gene in pBR322 the restriction endonuclease used can be