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Q.

In pBR322, the restriction site where insertional inactivation could not be achieved is

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a

BamH I

b

PvuI

c

Sal I

d

Cla I

answer is D.

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Detailed Solution

Cla I is the restriction site where insertional inactivation could not be achieved because it is not present in the region of marker genes. BamH I and Sal I are present in tetR marker region and Pvu I is present in ampR marker region.
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