First slide

Plant Growth Regulators

Question

Proliferation in callus can be attained by using auxins with
i- DNA or coconut milk
ii- Yeast extract
iii- Extracts from vascular tissues

Easy

A

i and ii only
B

i, ii and iii
C

ii and iii only
D

i and iii only

Solution

F. Skoog and his co-workers observed that from the internodal segments of tobacco stems the callus (a mass of undifferentiated cells) proliferated only if, in addition to auxins the nutrients medium was supplemented with one of the following: extracts of vascular tissues, yeast extract, coconut milk or DNA. Miller et al. (1955), later identified and crystallized the cytokinesis promoting active substance that they termed kinetin.

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