First slide

Tools of Recombinant DNA Technology

Question

Read the following statements and find the correct statement.

Easy

A

Escherichia coli is used to transfer T-DNA to transform normal plant cells into a tumor.
B

Gene gun is suitable for DNA fingerprinting.
C

pBR 322 has the restriction site for Sal I.
D

Restriction endonucleases cut or join DNA fragments.

Solution

pBR 322 contain the restriction endonuclease site for Hind III, EcoR I, BamH I, Sal I, Pvu II, Pst I, Cla I.
Restriction endonucleases cleaves DNA at specific site.
Agrobacterium tumefaciens is used to transfer T-DNA to transform normal plant cells into a tumor.
Gene gun is suitable for plants where cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA to directly introduce the alien DNA into the host cell.

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