First slide

Tools of Recombinant DNA Technology

Question

In recombinant DNA technology for the insertion of a foreign gene into a plasmid vector, the vector must be opened by treating it with

Easy

A

Alkaline phosphatase only
B

RNA ligase
C

DNA polymerase
D

The same restriction enzyme used to cleave the foreign DNA

Solution

When cut by the same restriction enzyme, the resultant DNA fragments of plasmid vector and foreign DNA have the same kind of ‘sticky-ends’ which can be joined together (end-to-end) using DNA ligases.

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