First slide

Tools of Recombinant DNA Technology

Question

Restriction endonuclease (EcoRI) enzyme cut the strand of DNA

Easy

A

from the centre of the palindromic sequence.
B

a little away from the centre of the palindromic sequence.
C

a little away from recognition site.
D

cuts only single-stranded DNA.

Solution

Each restriction endonuclease recognises a specific palindromic nucleotide sequences in the DNA. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double
helix at specific points in their sugar -phosphate backbones.

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