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a
Taq polymerase- Annealing of DNA
b
Chitinase - Obtaining DNA by lysis fungal cell
c
Exonucleases- Remove nucleotides from the ends of DNA
d
DNA ligase - Joining the fragments of DNA
answer is A.
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Detailed Solution
During PCR repeated amplification is achieved by the use of a thermostable DNA polymerase called Taq polymerase which is isolated from a bacterium, Thermus aquaticus, which remain active during the high temperature induced denaturation of double stranded DNA.