First slide

Tools of Recombinant DNA Technology

Question

The specific palindromic sequence which is recognized by EcoRI is :

Easy

A

5’-GGAACC-3’
3’-CCTTGG-5’
B

5’-CTTAAG-3’
3’-GAATTC-5’
C

5’-GGATCC-3’
3’-CCTAGG-5’
D

5’-GAATTC-3’
3’-CTTAAG-5’

Solution

Recognition sequence of RE EcoRI is $5' GAATTC 3' 3' CTTAAG 5'$
The palindrome in DNA is a sequence of base pairs that reads same on the two strands when orientation of reading is kept the same.
Recognition sequence of RE BamHI is 5’-GGATCC-3’
3’-CCTAGG-5’

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