Q.
The specific palindromic sequence which is recognized by EcoRI is :
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a
5’-GGAACC-3’3’-CCTTGG-5’
b
5’-CTTAAG-3’3’-GAATTC-5’
c
5’-GGATCC-3’3’-CCTAGG-5’
d
5’-GAATTC-3’3’-CTTAAG-5’
answer is D.
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Detailed Solution
Recognition sequence of RE EcoRI is 5'GAATTC3' 3'CTTAAG5' The palindrome in DNA is a sequence of base pairs that reads same on the two strands when orientation of reading is kept the same.Recognition sequence of RE BamHI is 5’-GGATCC-3’ 3’-CCTAGG-5’
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