Study the following gene cloning vector and identify the correct statements.I. Cloning sites are absent in ori region II. tetRhas restriction site for BamH I and Sal IIII. It is reconstructed cloning vector IV. It has two marker genes.

Cloning sites should be absent at the ori site because if gene is inserted at ori site, DNA replication is prevented. The marker tetR has cloning sites BamH I and Sal I. It has two marker genes such as ampR and tetR. It is a reconstructed vector because it is constructed from three plasmid sources. They are pSC101 from which tetR is obtained, Ori gene is obtained from Col E plasmid and ampicillin resistance gene is obtained from Col E1 plasmid