What are the requirements in tissue culture?
a. Hormones like auxin, cytokinin, agar-agar
b. Inorganic salt, vitamin, amino acid only
c. Carbon source like sucrose only
The requirements of tissue culture are growth hormones (auxin, cytokinin), agar-agar, inorganic salts, vitamins, amino acids, carbon source like sucrose, etc. In plants tissue culture, the medium on which explants are cultured is known as nutrient or culture medium. It acts as a source of important nutrient required for proper development of explant.