What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?
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a
The smaller the fragment size, the farther it moves.
b
Positively charged fragments move to farther end.
c
Negatively charged fragments do not move.
d
The larger the fragment size, the farther it moves.
answer is A.
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Detailed Solution
During gel electrophoresis DNA fragments separate (resolve) according to their size through sieving effect provided by agarose gel. Hence, smaller the fragment size, the farther it moves.