First slide

Biotechnological Applications in Agriculture

Question

What triggers activation of protoxin to active toxin of Bacillus thuringiensis in boll worm?

Easy

A

Acidic pH of stomach
B

Body temperature
C

Moist surface of midgut
D

Alkaline pH of gut

Solution

Bacillus thuringiensis forms protein crystals during a particular phase of their growth. These crystals contain a toxic insecticidal protein. The Bt toxin protein exist as inactive protoxins but once an insect like bollworm ingest the inactive toxin, it is converted into an active form of toxin due to the alkaline pH of the gut which solubilise the crystals. The activated toxin binds to the surface of midgut epithelial cells and create pores that cause cell swelling and lysis and eventually cause death of the insect.

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