First slide
Tools of Recombinant DNA Technology
Question

What type of enzyme is used in recombinant DNA technology to split a specific sugar phosphate bond in each strand of a DNA double helix ?

Easy
Solution

The restriction enzyme cleaves sugar phosphate bonds in DNA.
An esterase enzyme cleaves ester bonds. 
The lipase enzyme breaks down fats. 
A ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond

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