First slide

Transcription

Question

What will be the sequence of mRNA produced by the following stretch of DNA ?
3' ATGCATGCATGCATG5' TEMPLATE STRAND
5' TACGTACGTACGTAC3' CODING STRAND

Easy

A

3'AUGCAUGCAUGCAUG5'
B

5'UACGUACGUACGUAC 3'
C

3' UACGUACGUACGUAC 5'
D

5' AUGCAUGCAUGCAUG 3'

Solution

DNA dependent RNA polymerase catalyze the polymerization in only one direction, that is, 5’→3’, the strand that has the polarity 3’→5’ acts as a template, and is also referred to as template strand.

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