When a foreign DNA is inserted within the tetracycline resistance marker gene of pBR322, it would result in:
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a
Insertional activation
b
Transformation
c
Insertional inactivation
d
Direct DNA insertion
answer is C.
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Detailed Solution
When a foreign DNA is inserted within the tetracycline resistance marker gene of pBR322, it would result in inactivation of the gene for synthesis of this tetracycline, which is referred to as insertional inactivation.