First slide

Tools of Recombinant DNA Technology

Question

Which amongst the following enzyme is required to remove nucleotides from the ends of the DNA ?

Easy

A

Endonuclease
B

Exonuclease
C

DNA ligase
D

DNA polymerase

Solution

Exonucleases remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA. DNA ligase is a specific type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. DNA polymerase, uses a DNA template to catalyse the polymerisation of deoxyribonucleotides.

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