Which of the following is not a characteristic feature of the vector pBR322?

• A

  It has two antibiotic - resistance genes : amp^R and tet^R.

• B

  It was the first artificial cloning vector constructed in 1997 by Boliver and Rodriguez.

• C

  The amplicillin-resistance gene has restriction sites for Bam HI and Sal I

• D

  The restriction site for Pvu II is present in the replication of plasmid (rop) gene.