Which of the following steps during glycolysis is associated with utilization of ATP?

• A

  Glucose → Glucose-6-phosphate only

• B

  Fructose-6-phosphate → Fructose-1-6-biphosphate only

• C

  PEP → Pyruvic acid

• D

  Both Glucose$\to$ Glucose-6-phosphate and Fructose-6-phosphate$\to$ Fructose-1,6-bisphosphate.