Biotechnological Applications in Agriculture
Question
Which of the following vector is used to introduce nematode specific genes into the host plant?
Easy
Solution
Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant. The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNAs being complementary to each other formed a double stranded RNA (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode. The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA.
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