First slide

The DNA

Question

In which of the following will DNA melt at the lowest temperature?

Easy

A

5’ – AATAAAGC – 3’
3’ – TTATTTCG – 5’
B

5’ – AATGCTGC – 3’
3’ – TTACGACG – 5’
C

5’ – ATGCTGAT – 3’
3’ – TACGACTA – 5’
D

5’ – GCATAGCT – 3’
3’ – CGTATCGA – 5’

Solution

5’ – AATAAAGC – 3’
3’ – TTATTTCG – 5’ has 18 hydrogen bonds and others have more hydrogen bonds. Hence DNA with this sequence melts at low temperatures compared to
5’ – AATGCTGC – 3’
3’ – TTACGACG – 5’
5’ – ATGCTGAT – 3’
3’ – TACGACTA – 5’ and
5’ – GCATAGCT – 3’
3’ – CGTATCGA – 5’

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