Questions
In which of the following will DNA melt at the lowest temperature?
a
5’ – AATAAAGC – 3’3’ – TTATTTCG – 5’
b
5’ – AATGCTGC – 3’3’ – TTACGACG – 5’
c
5’ – ATGCTGAT – 3’3’ – TACGACTA – 5’
d
5’ – GCATAGCT – 3’3’ – CGTATCGA – 5’
detailed solution
Correct option is A
5’ – AATAAAGC – 3’3’ – TTATTTCG – 5’ has 18 hydrogen bonds and others have more hydrogen bonds. Hence DNA with this sequence melts at low temperatures compared to5’ – AATGCTGC – 3’3’ – TTACGACG – 5’5’ – ATGCTGAT – 3’3’ – TACGACTA – 5’ and 5’ – GCATAGCT – 3’3’ – CGTATCGA – 5’Talk to our academic expert!
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