First slide

Tools of Recombinant DNA Technology

Question

Which gene is inserted as marker in screening recombinant transformants from non-recombinant transformants in a plasmid vector?

Easy

A

gene ‘i’
B

gene ‘y’
C

gene ‘z’
D

gene ‘a’

Solution

Gene z can be used as second marker instead of antibiotic resistance marker gene to identify the recombinant transformants which appear as white colonies from non-recombinant blue colonies, because the gene inserted in lac z region of vector cannot form active β-galactosidase that can convert the X-gal substrate into blue colored product and the colonies of non-recombinants appear blue.

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