First slide

Tissue Culture

Question

You are given a tissue with its potential for differentiation in an artificial culture. Which of the following pairs of hormones would you add to the medium to secure shoots as well as roots?

Easy

A

IAA and gibberellin
B

Auxin and cytokinin
C

Auxin and abscisic acid
D

Gibberellin and abscisic acid

Solution

During tissue culture. Organogenesis is one of crucial step. Cytokinin / Auxin ratio is one of the method of organogenesis.
$Callus \overset{High C / A}{\to} Shoot \overset{Low C / A}{\to}$ Root on shoot
Hence adjustment of C/A leads to development of both shoot and Root.
GA does not have any role in Morphogenesis
ABA is growth inhibitor so can’t promote organogenesis

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