Q.
You are given a tissue with its potential for differentiation in an artificial culture. Which of the following pairs of hormones would you add to the medium to secure shoots as well as roots?
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a
IAA and gibberellin
b
Auxin and cytokinin
c
Auxin and abscisic acid
d
Gibberellin and abscisic acid
answer is B.
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Detailed Solution
During tissue culture. Organogenesis is one of crucial step. Cytokinin / Auxin ratio is one of the method of organogenesis.Callus→High C/A Shoot→Low C/ARoot on shootHence adjustment of C/A leads to development of both shoot and Root.GA does not have any role in MorphogenesisABA is growth inhibitor so can’t promote organogenesis
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