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Q.

You are given a tissue with its potential for differentiation in an artificial culture. Which of the following pairs of hormones would you add to the medium to secure shoots as well as roots?

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a

IAA and gibberellin

b

Auxin and cytokinin

c

Auxin and abscisic acid

d

Gibberellin and abscisic acid

answer is B.

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Detailed Solution

During tissue culture. Organogenesis is one of crucial step. Cytokinin / Auxin ratio is one of the method of organogenesis.Callus→High C/A  Shoot→Low C/ARoot on shootHence adjustment of C/A leads to development of both shoot and Root.GA does not have any role in MorphogenesisABA is growth inhibitor so can’t promote organogenesis
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