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A solution of crab haemocyanin, a pigmented protein extracted from crabs, was prepared by dissolving 0.750 g in 125 cm3 of an aqueous medium. At 4oC an osmotic pressure rise of 2.6 mm of the solution was observed. The solution has a density of 1.00 g/cm3. Determine the molecular weight of the protein.

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Detailed Solution

(0.75m)125×(8.314×105×102)(277)=2.6×10−2(1)(1000)m=(0.75)(8.314×107)(277)125×26m=53.14×105 g/mol

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A solution of crab haemocyanin, a pigmented protein extracted from crabs, was prepared by dissolving 0.750 g in 125 cm3 of an aqueous medium. At 4oC an osmotic pressure rise of 2.6 mm of the solution was observed. The solution has a density of 1.00 g/cm3. Determine the molecular weight of the protein.