The membrane that separates scala media and scala tympani is

Cochlea is divided into three chambers; upper scala vestibuli, middle scala media and lower scala tympani. Reissner's membrane is between scala vestibuli and scala media. Basilar membrane is between scala media and scala tympani.Tectorial membrane is a thin elastic membrane over a row of hair cells in the organ of corti.Tympanic membrane separates external ear and the middle ear.