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NCERT Solutions for class 12 Biology chapter 11 biotechnology principles and processes 11.3 processes of recombinant dna technology

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NCERT Solutions for Class 12 Biology Chapter 11 Topic- Processes of Recombinant DNA Technology

NCERT Solutions for Class 12 Biology Chapter 11 Topic- Processes of Recombinant DNA Technology and all chapter Topics produced by qualified teachers according to NCERT (CBSE) book rules are available as a free PDF download. Class 12 Biology Chapter 11 Topic- Processes of Recombinant DNA Technology Questions with Solutions to assist you in revising the entire syllabus and achieving higher grades. Register to receive all exercise solutions through email.

Solutions

Overview of topic

DNA extraction

Isolation of DNA, which is a nucleic acid encased within the nucleus, is a difficult task. Isolation of DNA is an enzymatically regulated technique that involves the treatment of plant or animal cells with certain enzymes. To isolate pure DNA from cells, enzymes such as cellulase (plant cells), lysozyme (bacteria), and chitinase (fungi) are utilized.

DNA fragmentation is a phenomenon that occurs when a person’s DNA is broken up

Restriction endonucleases are used to cleave the DNA into pieces once it has been extracted and purified. The restriction enzymes used in recombinant DNA technology are critical for detecting the precise position where the desired gene is inserted into the vector genome.

Gene of Interest Amplification

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    After restriction enzymes have been used to cut the correct gene of interest, the polymerase chain reaction (PCR) is used to amplify the gene. Multiple copies of the gene of interest can be made using this method. Denaturation, annealing, and extension are the three phases of PCR.

    Recombinant DNA is inserted into the host.

    The host is the rDNA technology’s last tool, which uses enzymes to devour the vector encoded with the required DNA. The intended recombinant DNA can be inserted into the host organism in a variety of methods. Biolistics or the gene gun, microinjection, alternate heating and cooling, calcium ion use, and so forth are all examples of this. The recombinant DNA test is passed by the effectively transformed cells or organisms.

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    FAQs (Frequently Asked Questions)

    1. Can I score full marks in Class 12 Chapter 11 Biology?

    Yes, you can easily score full marks in Class 12 Biology. All you need to do to score full marks in Biology is frequent practice. All of the concepts should be clear in this subject since many questions are expected in the board examination from this chapter. This will help you make your confidence up and well versed with the chapter and can score full marks in the exam. You should also refer to the NCERT Solutions of this chapter.

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